Comparative Study of Biosurfactant Production by Saccharomyces Cerevisiae Using Various Carbon Sources
Keywords:
Biosurfactant, Carbon sources, Oils, Crude oil, Olive oil
Abstract
Saccharomyces cerevisiae, a widely used and safe yeast in biotechnology, was evaluated for its ability to produce an environmentally friendly biosurfactant using various carbon sources. This study aimed to compare biosurfactant production from glucose, crude oil, olive oil, and cooking oil, addressing the gap in understanding the impact of insoluble carbon sources. Yeast was activated in YEPG broth and cultured in modified YEP medium with different carbon sources. The biosurfactant activity was assessed through surface tension reduction (ST) and oil spreading method (OSM). Results indicated that glucose did not significantly enhance biosurfactant production (60mN/m ST, 3mm OSM), while crude oil (50mN/m ST, 45mm OSM), olive oil (53mN/m ST, 40mm OSM), and cooking oil (54mN/m ST, 35mm OSM) significantly improved activity. The findings demonstrate that insoluble carbon sources are crucial for effective biosurfactant production by S. cerevisiae, with crude oil yielding the most active biosurfactant. This research highlights the potential of using oils to enhance biosurfactant production, providing insights for industrial applications.
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H. Dhivya, S. Balaji, and A. Madhan Rand Srv, "Production of Amphiphilic Surfactant Molecule from Saccharomyces Cerevisiae Mtcc 181 and its Protagonist in Nanovesicle Synthesis," *International Journal of Pharmaceutical Sciences*, vol. 11, pp. 16-23, 2014.
L. Derguine-Mecheri, S. Kebbouche-Gana, and D. Djenane, "Biosurfactant Production from Newly Isolated Rhodotorula sp. YBR and its Great Potential in Enhanced Removal of Hydrocarbons from Contaminated Soils," *World Journal of Microbiology and Biotechnology*, vol. 37, 2021.
M. Abouseoud, R. Maachi, and A. Amrane, "Biosurfactant Production from Olive Oil by Pseudomonas Fluorescens," *Communicating Current Research and Educational Topics and Trends in Applied Microbiology*, vol. 1, pp. 340-347, 2007.
D. G. Almeida, R. D. S. Silva, H. M. Meira, P. P. F. Brasileiro, E. J. Silva, J. M. Luna, et al., "Production, Characterization and Commercial Formulation of a Biosurfactant from Candida Tropicalis UCP0996 and its Application in Decontamination of Petroleum Pollutants," *Processes*, vol. 9, no. 5, p. 885, 2021.
T. F. Ferreira, F. F. Martins, C. A. Cayres, P. F. Amaral, D. D. A. Azevedo, and M. A. Z. Coelho, "Biosurfactant Production from the Biodegradation of n-Paraffins, Isoprenoids and Aromatic Hydrocarbons from Crude Petroleum by Yarrowia Lipolytica IMUFRJ 50682," *Fermentation*, vol. 9, no. 1, p. 21, 2022.
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D. H. Raham and N. N. Mahmood, "Isolation of Biosurfactant/Bioemulsifier from Saccharomyces Cerevisiae by Different Treatment," *Al-Kufa University Journal for Biology*, vol. 9, no. 3, pp. 22-28, 2017.
Published
2024-05-16
How to Cite
Ibrahim, Z. A., Hussein, A. A., & Khudhair, S. H. (2024). Comparative Study of Biosurfactant Production by Saccharomyces Cerevisiae Using Various Carbon Sources. Central Asian Journal of Medical and Natural Science, 5(3), 324-330. Retrieved from https://www.cajmns.centralasianstudies.org/index.php/CAJMNS/article/view/2445
Section
Articles
Copyright (c) 2024 Zeena A. Ibrahim, Amal A. Hussein, Saad H. Khudhair
This work is licensed under a Creative Commons Attribution 4.0 International License.
