Role of MIF and HSP70 Proteins in Immune Response for Patients Infected with Toxoplasmosis
Keywords:
Toxoplasma gondii, MIF, HSP70, Babil
Abstract
The study was conducted on 250 suspected patients and 30 healthy persons who have visited Al-Marjan Hospital in Babil province from September 2021 to till February 2022. This study aimed to evaluation the serum levels of the MIF cytokine and HSP70 protein in females with Toxoplasmosis and control groups. The parasite was recognized after an examination of the Serum sample by using a VIDAS® TOXO IgG II. A total of 250 suspected patients were collected, all of these are females, sixty out of 250 (24 %) 60 females are patients infected with Toxoplasmosis and 30 healthy peoples. The results of current study showed higher significantly P˂0.05 in concentration MIF cytokine in patients group (45.52±21.71) ng/ml compared with control group with concentration (16.48±6.86). The concentration of HSP70 level was higher significantly P˂0.05 in patients group (3.70±3.06) ng/ml than in control group (1.61±0.92) ng/ml.
References
1. Al-abodi, H.J (2019). Use of immunological methods to the detection of toxoplasmosis and heat shock protein HSP70 in men. J parasite Dis. 43(2): 234-239.
2. Al-Hadraawy S. K.; Al-Hadraawy M. K.; Ali H. A; Hydar M. K. (2016). Evaluation levels of immunoglobin g and lipid profile in patients infected with hydatid cyst in Al-Najaf governate, Iraq. Asian Jr. of Microbiol. Biotech. Env. Sc. 18 (1): 101-104.
3. AL-Ubaydi N.A.H. (2011) Investigation of the heat shock protein HPS70 among women infected with T. gondii parasitic infections in Thi-Qar Governorate. In: Proceeding of 4th international scientific conference, College of education for pure sciences, University of Thi-Qar.
4. Barenco, P. V. C., Lourenco, E. V., Cunha-Junior, J. P., Almeida, K. C., Roque-Barreira, M. C., Silva, D. A. O.,. . & Silva, N. M. (2014).Toxoplasma gondii 70 kDa Heat Shock Protein: Systemic Detection Is Associated with the Death of the Parasites by the Immune Response and Its Increased Expression in the Brain Is Associated with Parasite Replication. Plosone.org. Vol 9. Issue 5.e96527.
5. Bolhassani, A., & Agi, E. (2019). Heat shock proteins in infection. Clinica Chimica Acta, 498, 90-100.
6. Bukau, B.; Weissman, J.; Horwich, A. Molecular chaperones and protein quality control. Cell 2016, 125, 443–451.
7. Cabral, F.J.; Vianna, L.G.; Medeiros, M.M.; Carlos, B.C.; Martha, R.D.; Silva, N.M.; Da Silva, L.H.P.; Stabeli, R.G.; Wunderlich, G. Immunoproteomics of Plasmodium falciparum-infected red blood cell membrane fractions. Mem. Inst. Oswaldo Cruz 2017, 112, 850–856.
8. Calandra T. and Roger T. (2003). Macrophage migration inhibitory factor: a regulator of innate immunity. Nature Reviews Immunology, 3(10): 791–800.
9. Ghosh S.; Padalia J.; Ngobeni R.; Abendroth J.; Farr L.; Shirley D.-A.; Edwards T. and Moonah S. (2020). Targeting Parasite-Produced Macrophage Migration Inhibitory Factor as an Antivirulence Strategy With Antibiotic–Antibody Combination to Reduce Tissue Damage. The Journal of Infectious Diseases, 221(7): 1185–1193.
10. Ghosh S.; Leaton L. A.; Farr L.; Barfield A. and Moonah S. (2018). Interaction between parasite-encoded JAB1/CSN5 and macrophage migration inhibitory factor proteins attenuates its proinflammatory function. Scientific Reports, 8(1): 1–9.
11. Ghosh S.; Leaton L. A.; Farr L.; Barfield A. and Moonah S. (2018). Interaction between parasite-encoded JAB1/CSN5 and macrophage migration inhibitory factor proteins attenuates its proinflammatory function. Scientific Reports, 8(1): 1–9.
12. Grieb G. Merk; M. Bernhagen J. and Bucala R. (2010). Macrophage migration inhibitory factor (MIF): a promising biomarker. Drug News & Perspectives, 23(4): 257.
13. Hertelendy J.; Reumuth G.; Simons D.; Stoppe C.; Kim B. S.; Stromps J. P. (2018). Macrophage migration inhibitory factor-afavorable markerin inflammatory diseases? Curr.Med. Chem. 25: 601-605.
14. Hertelendy J.; Reumuth G.; Simons D.; Stoppe C.; Kim B. S.; Stromps J. P. (2018). Macrophage migration inhibitory factor-afavorable markerin inflammatory diseases? Curr. Med. Chem. 25: 601-605.
15. Hideyuki, N. A. G. (2002). Host Immune system against Toxoplasma infection, national research center for protozoan Diseases, Jpn.J. Protozool. Vol. 35 (1): 080 – 8555.
16. Hill, D., & Dubey, J. P. (2002). Toxoplasma gondii: transmission, diagnosis and prevention. Clinical microbiology and infection, 8(10), 634-640.
17. Hofmann S, Batz MB, Morris JG Jr. Annual cost of illness and qualityadjusted life year losses in the United States due to 14 foodborne pathogens. J Food Prot. 2012; 75:1292–302.
18. Hotez PJ. Neglected parasitic infections and poverty in the United States. PLoS Negl Trop Dis. 2014; 8: e3012.
19. Hussian, A. R. (2022). Study role NLRP3 and some immunological markers in patients infected with Toxoplasma gondii parasite. Al-Najaf province. MSc. thesis, Faculty of Science. University of Kufa.
20. Jones J.L.; Parise M.E. and Fiore A.E. (2014). Neglected parasitic infections in the United States, Toxoplasmosis. Am J. Trop. Med.Hyg. 90:794–799.
21. Kang I. and Bucala R. (2019). The immune biology of MIF: Function, genetics and prospects for precision medicine. Nature Reviews Rheumatology, 15(7): 427–437.
22. Khan, I. A., & Moretto, M. (2022). Immune responses to Toxoplasma gondii. Current Opinion in Immunology, 77, 102226.
23. Kozaci D. L.; Ertuğ S.; Kavak T.; Okyay P.; Chikanza I. C. and Ertabaklar H. (2005). Investigation of Serum Macrophage Migration Inhibitor Factor (MIF) levels in patients with cutaneous leishmaniasis. Turkiye Parazitolojii Dergisi, 29(3): 145–148.
24. Montoya, J. G., and Liesenfeld, O. (2004). Toxoplasmosis. Lancet 363, 1965–1976. doi: 10.1016/S0140-6736(04)16412-X.
25. Moonah S.; Ghosh S.; Jiang N.; Farr L. and Ngobeni R. (2019). Parasite-produced MIF cytokine: role in immune evasion, invasion, and pathogenesis. Frontiers in Immunology, 10, 1995.
26. Moonah, S. N.; Abhyankar M. M.; Haque, R. and Petri ,W. A. (2014). The macrophage migration inhibitory factor homolog of Entamoeba histolytica binds to and immunomodulates host macrophages. Infection and Immunity, 82(9):3523–3530.
27. Moonah, S. N.; Jiang, N. M. and Petri, Jr. W. A. (2013). Host immune response to intestinal amebiasis. PLoS.Pathog, 9(8): e1003489.
28. Multhoff, G.; Hightower, L.E. Distinguishing integral and receptor-bound heat shock protein 70 (Hsp70) on the cell surface by Hsp70-specific antibodies. Cell Stress Chaperones 2011, 16, 251–255.
29. Ngobeni R.; Abhyankar M. M.; Jiang N. M.; Farr L. A.; Samie A.; Haque R. and Moonah S. N. (2017). Entamoeba histolytica–Encoded Homolog of Macrophage Migration Inhibitory Factor Contributes to Mucosal Inflammation during Amebic Colitis. The Journal of Infectious Diseases, 215(8): 1294–1302.
30. Peyron, F, Wallon, M, Kieffer, F and Graweg, G (2016). Toxoplasmosis. In: Remington J.S Klein J.O., Wilson C.B., Nizet, V., Maldonado, Y.A. (8th eds.) Infectious diseases of the fetus and newborn infant. Philadelphia, USA: Elsevier Saunders, pp. 949-1042.
31. Pomares, C. and Montoya, JG. Laboratory Diagnosis of Congenital Toxoplasmosis. J Clin Microbiol. (2016). 54(10):2448–54.
32. Rodrigues Oliveira J. L.; Teixeira M. M.; Lambertucci, J. R.; Antunes, C. M. F.; Carneiro, M. and Negrão; Corrêa, D. (2018). Plasma levels of innate immune mediators are associated with liver fibrosis in low parasite burden Schistosoma mansoni infected individuals. Scandinavian Journal of Immunology, 87(3):e12642.
33. Sasai, M., and Yamamoto, M. (2019). Innate, adaptive, and cell-autonomous immunity against Toxoplasma gondii infection. Experimental & molecular medicine, 51(12), 1-10.
34. Sierra Puente, R. E.; CAMPOS‐RODRÍGUEZ, R.; JARILLO‐LUNA R. A.; Muñoz‐Fernández L.; Rodríguez M. G.; Muñoz‐Ortega M. H. and Ventura‐Juárez J. (2009). Expression of immune modulator cytokines in human fulminant amoebic colitis. Parasite Immunology, 31(7): 384–.
35. Tenter, A. M., Heckeroth, A. R., and Weiss, L. M. (2000). Toxoplasma gondii: from animals to humans. Int. J. Parasitol. 30, 1217–1258. doi: 10.1016/S0020-7519(00)00124-7.
36. Vaillant, V., Valk, H. D., Baron, E., Ancelle, T., Colin, P., Delmas, M.-C., et al. (2005). Foodborne infections in France. Foodborne Pathog. Dis. 2, 221–232. doi: 10.1089/fpd.2005.2.221.
37. Valencia-Pacheco, G.; Loría-Cervera, E. N.; Sosa-Bibiano, E. I.; Canché-Pool, E. B.; Vargas-Gonzalez, A.; Melby, P. C. and Andrade-Narvaez, F. J. 2014. In situ cytokines (IL-4, IL-10, IL-12, IFN-γ) and chemokines (MCP-1, MIP-1α) gene expression in human Leishmania (Leishmania) mexicana infection. cytokine. 69(1): 56-61.
38. Yoo S.-A.; Leng L.; Kim B.-J.; Du X.; Tilstam P. V.; Kim K. H.; Kong J.-S.; Yoon H.-J.; Liu A. and Wang T. (2016). MIF allele-dependent regulation of the MIF coreceptor CD44 and role in rheumatoid arthritis. Proceedings of the National Academy of Sciences, 113(49): E7917–E7926.
39. Zininga T.; Ramatsui L. and Shonhai A. (2018). Heat Shock Proteins as Immunomodulants. Molecules. 23(11): 2846.
2. Al-Hadraawy S. K.; Al-Hadraawy M. K.; Ali H. A; Hydar M. K. (2016). Evaluation levels of immunoglobin g and lipid profile in patients infected with hydatid cyst in Al-Najaf governate, Iraq. Asian Jr. of Microbiol. Biotech. Env. Sc. 18 (1): 101-104.
3. AL-Ubaydi N.A.H. (2011) Investigation of the heat shock protein HPS70 among women infected with T. gondii parasitic infections in Thi-Qar Governorate. In: Proceeding of 4th international scientific conference, College of education for pure sciences, University of Thi-Qar.
4. Barenco, P. V. C., Lourenco, E. V., Cunha-Junior, J. P., Almeida, K. C., Roque-Barreira, M. C., Silva, D. A. O.,. . & Silva, N. M. (2014).Toxoplasma gondii 70 kDa Heat Shock Protein: Systemic Detection Is Associated with the Death of the Parasites by the Immune Response and Its Increased Expression in the Brain Is Associated with Parasite Replication. Plosone.org. Vol 9. Issue 5.e96527.
5. Bolhassani, A., & Agi, E. (2019). Heat shock proteins in infection. Clinica Chimica Acta, 498, 90-100.
6. Bukau, B.; Weissman, J.; Horwich, A. Molecular chaperones and protein quality control. Cell 2016, 125, 443–451.
7. Cabral, F.J.; Vianna, L.G.; Medeiros, M.M.; Carlos, B.C.; Martha, R.D.; Silva, N.M.; Da Silva, L.H.P.; Stabeli, R.G.; Wunderlich, G. Immunoproteomics of Plasmodium falciparum-infected red blood cell membrane fractions. Mem. Inst. Oswaldo Cruz 2017, 112, 850–856.
8. Calandra T. and Roger T. (2003). Macrophage migration inhibitory factor: a regulator of innate immunity. Nature Reviews Immunology, 3(10): 791–800.
9. Ghosh S.; Padalia J.; Ngobeni R.; Abendroth J.; Farr L.; Shirley D.-A.; Edwards T. and Moonah S. (2020). Targeting Parasite-Produced Macrophage Migration Inhibitory Factor as an Antivirulence Strategy With Antibiotic–Antibody Combination to Reduce Tissue Damage. The Journal of Infectious Diseases, 221(7): 1185–1193.
10. Ghosh S.; Leaton L. A.; Farr L.; Barfield A. and Moonah S. (2018). Interaction between parasite-encoded JAB1/CSN5 and macrophage migration inhibitory factor proteins attenuates its proinflammatory function. Scientific Reports, 8(1): 1–9.
11. Ghosh S.; Leaton L. A.; Farr L.; Barfield A. and Moonah S. (2018). Interaction between parasite-encoded JAB1/CSN5 and macrophage migration inhibitory factor proteins attenuates its proinflammatory function. Scientific Reports, 8(1): 1–9.
12. Grieb G. Merk; M. Bernhagen J. and Bucala R. (2010). Macrophage migration inhibitory factor (MIF): a promising biomarker. Drug News & Perspectives, 23(4): 257.
13. Hertelendy J.; Reumuth G.; Simons D.; Stoppe C.; Kim B. S.; Stromps J. P. (2018). Macrophage migration inhibitory factor-afavorable markerin inflammatory diseases? Curr.Med. Chem. 25: 601-605.
14. Hertelendy J.; Reumuth G.; Simons D.; Stoppe C.; Kim B. S.; Stromps J. P. (2018). Macrophage migration inhibitory factor-afavorable markerin inflammatory diseases? Curr. Med. Chem. 25: 601-605.
15. Hideyuki, N. A. G. (2002). Host Immune system against Toxoplasma infection, national research center for protozoan Diseases, Jpn.J. Protozool. Vol. 35 (1): 080 – 8555.
16. Hill, D., & Dubey, J. P. (2002). Toxoplasma gondii: transmission, diagnosis and prevention. Clinical microbiology and infection, 8(10), 634-640.
17. Hofmann S, Batz MB, Morris JG Jr. Annual cost of illness and qualityadjusted life year losses in the United States due to 14 foodborne pathogens. J Food Prot. 2012; 75:1292–302.
18. Hotez PJ. Neglected parasitic infections and poverty in the United States. PLoS Negl Trop Dis. 2014; 8: e3012.
19. Hussian, A. R. (2022). Study role NLRP3 and some immunological markers in patients infected with Toxoplasma gondii parasite. Al-Najaf province. MSc. thesis, Faculty of Science. University of Kufa.
20. Jones J.L.; Parise M.E. and Fiore A.E. (2014). Neglected parasitic infections in the United States, Toxoplasmosis. Am J. Trop. Med.Hyg. 90:794–799.
21. Kang I. and Bucala R. (2019). The immune biology of MIF: Function, genetics and prospects for precision medicine. Nature Reviews Rheumatology, 15(7): 427–437.
22. Khan, I. A., & Moretto, M. (2022). Immune responses to Toxoplasma gondii. Current Opinion in Immunology, 77, 102226.
23. Kozaci D. L.; Ertuğ S.; Kavak T.; Okyay P.; Chikanza I. C. and Ertabaklar H. (2005). Investigation of Serum Macrophage Migration Inhibitor Factor (MIF) levels in patients with cutaneous leishmaniasis. Turkiye Parazitolojii Dergisi, 29(3): 145–148.
24. Montoya, J. G., and Liesenfeld, O. (2004). Toxoplasmosis. Lancet 363, 1965–1976. doi: 10.1016/S0140-6736(04)16412-X.
25. Moonah S.; Ghosh S.; Jiang N.; Farr L. and Ngobeni R. (2019). Parasite-produced MIF cytokine: role in immune evasion, invasion, and pathogenesis. Frontiers in Immunology, 10, 1995.
26. Moonah, S. N.; Abhyankar M. M.; Haque, R. and Petri ,W. A. (2014). The macrophage migration inhibitory factor homolog of Entamoeba histolytica binds to and immunomodulates host macrophages. Infection and Immunity, 82(9):3523–3530.
27. Moonah, S. N.; Jiang, N. M. and Petri, Jr. W. A. (2013). Host immune response to intestinal amebiasis. PLoS.Pathog, 9(8): e1003489.
28. Multhoff, G.; Hightower, L.E. Distinguishing integral and receptor-bound heat shock protein 70 (Hsp70) on the cell surface by Hsp70-specific antibodies. Cell Stress Chaperones 2011, 16, 251–255.
29. Ngobeni R.; Abhyankar M. M.; Jiang N. M.; Farr L. A.; Samie A.; Haque R. and Moonah S. N. (2017). Entamoeba histolytica–Encoded Homolog of Macrophage Migration Inhibitory Factor Contributes to Mucosal Inflammation during Amebic Colitis. The Journal of Infectious Diseases, 215(8): 1294–1302.
30. Peyron, F, Wallon, M, Kieffer, F and Graweg, G (2016). Toxoplasmosis. In: Remington J.S Klein J.O., Wilson C.B., Nizet, V., Maldonado, Y.A. (8th eds.) Infectious diseases of the fetus and newborn infant. Philadelphia, USA: Elsevier Saunders, pp. 949-1042.
31. Pomares, C. and Montoya, JG. Laboratory Diagnosis of Congenital Toxoplasmosis. J Clin Microbiol. (2016). 54(10):2448–54.
32. Rodrigues Oliveira J. L.; Teixeira M. M.; Lambertucci, J. R.; Antunes, C. M. F.; Carneiro, M. and Negrão; Corrêa, D. (2018). Plasma levels of innate immune mediators are associated with liver fibrosis in low parasite burden Schistosoma mansoni infected individuals. Scandinavian Journal of Immunology, 87(3):e12642.
33. Sasai, M., and Yamamoto, M. (2019). Innate, adaptive, and cell-autonomous immunity against Toxoplasma gondii infection. Experimental & molecular medicine, 51(12), 1-10.
34. Sierra Puente, R. E.; CAMPOS‐RODRÍGUEZ, R.; JARILLO‐LUNA R. A.; Muñoz‐Fernández L.; Rodríguez M. G.; Muñoz‐Ortega M. H. and Ventura‐Juárez J. (2009). Expression of immune modulator cytokines in human fulminant amoebic colitis. Parasite Immunology, 31(7): 384–.
35. Tenter, A. M., Heckeroth, A. R., and Weiss, L. M. (2000). Toxoplasma gondii: from animals to humans. Int. J. Parasitol. 30, 1217–1258. doi: 10.1016/S0020-7519(00)00124-7.
36. Vaillant, V., Valk, H. D., Baron, E., Ancelle, T., Colin, P., Delmas, M.-C., et al. (2005). Foodborne infections in France. Foodborne Pathog. Dis. 2, 221–232. doi: 10.1089/fpd.2005.2.221.
37. Valencia-Pacheco, G.; Loría-Cervera, E. N.; Sosa-Bibiano, E. I.; Canché-Pool, E. B.; Vargas-Gonzalez, A.; Melby, P. C. and Andrade-Narvaez, F. J. 2014. In situ cytokines (IL-4, IL-10, IL-12, IFN-γ) and chemokines (MCP-1, MIP-1α) gene expression in human Leishmania (Leishmania) mexicana infection. cytokine. 69(1): 56-61.
38. Yoo S.-A.; Leng L.; Kim B.-J.; Du X.; Tilstam P. V.; Kim K. H.; Kong J.-S.; Yoon H.-J.; Liu A. and Wang T. (2016). MIF allele-dependent regulation of the MIF coreceptor CD44 and role in rheumatoid arthritis. Proceedings of the National Academy of Sciences, 113(49): E7917–E7926.
39. Zininga T.; Ramatsui L. and Shonhai A. (2018). Heat Shock Proteins as Immunomodulants. Molecules. 23(11): 2846.
Published
2023-12-27
How to Cite
Ali, L. H., & Muslim, W. A. (2023). Role of MIF and HSP70 Proteins in Immune Response for Patients Infected with Toxoplasmosis. Central Asian Journal of Medical and Natural Science, 4(6), 1273-1280. Retrieved from https://www.cajmns.centralasianstudies.org/index.php/CAJMNS/article/view/2226
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Articles
