ANALYSIS OF THE SIGNIFICANCE OF POLYMORPHIC LOCUSES OF THE IL17A (rs2275913) AND IL17F (rs763780) GENE IN AUTOIMMUNE HEMOLYTIC ANEMIA

Jakhonov A.Kh.; Matkarimova D.S.; Saidov A.; Boboev K.T.

Jakhonov A.Kh.
Matkarimova D.S.
Saidov A.
Boboev K.T.

Keywords: IL17A (rs2275913), IL-17F (rs763780), autoimmune hemolytic anemia, carriage, frequency, allele, genotype, risk of development, severe course

Abstract

Relevance. Autoimmune hemolytic anemia (AIHA), an acquired and relatively rare pathology in the entire group of hematological diseases, is characterized by the formation of autoantibodies, which in most cases leads to premature, in most cases intracellular, hemolysis of red blood cells.

Target. To study the features of the distribution of polymorphic loci of the IL17A (rs2275913) and IL17F (rs763780) genes in patients with AIHA, and also to determine their significance in the development of the disease and its severe course.

Methods and materials.The study included 93 adult patients (main group) diagnosed with autoimmune hemolytic anemia and 97 healthy donors (control comparison group) with no history of autoimmune diseases, matched by gender and age to the main group of patients.

Detection of the IL17A (rs2275913) and IL17F (rs763780) genetic polymorphisms was carried out by SNP-PCR using an Applied Biosystems 2720 (USA) programmable thermocycler using test systems from Litech (Russia) and Syntol (Russia).Statistical analysis of the results was carried out using the statistical software package “OpenEpi 2009, Version 9.3”.

Results.When assessing the nature of the differences in the distribution of alleles and genotypes of the cytokine polymorphic gene interleukin IL17A (rs2275913) in the main group of patients with AIHA compared to healthy patients, it was found that carriers of a weakened allele (A) and genotypes are present are (G/A and A/A) is 2.1 times (χ2=7.9; P=0.01), 1.8 times (χ2=3.1; P=0, 1) and 6.6-fold (χ2=3.9; p=0.1) increased risk for the formation of AIHA. P=0.05) as well as with an increased risk of formation of this form of AIHA by 3.0 times (χ2=12.0; P=0.01), 2.2 times (χ2=3.9 ; P=0.05) and 11.3-fold (χ2). =6.9; P=0.01).

Statistical calculations of the distribution of polymorphic loci of the cytokine polymorphic gene interleukin IL-17F (rs763780) in patient groups compared to healthy patients made it possible to establish the presence of a tendency to increase the risk of developing AIHA in carriers of the mutant Arg allele almost twice as much (χ2 = 2.7; P = 0.2) and His/Arg heterozygous carriers twice as much (20.4% versus 11.3%; χ2 = 3.0; P = 0.1; OR = 2.0; CI: 0.91–4.45) and a trend toward a 2.2-fold (χ2=3.0; P=0.1) and 2.4-fold (χ2=3.3; P=0.1) increased risk for development, respectively severe AIHA when unfavorable alleles (Arg) and genotype (His/Arg) are carried, respectively.

Thus, the results show the possible contribution of the polymorphic genes IL17A (rs2275913) and IL-17F (rs763780) to the mechanisms of AIHA development and the development of severe diseases.

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