FREQUENCY AND SENSITIVITY OF PROTEUS SPP, PSEUDOMONAS SPP, AND STAPHYLOCOCCUS SPP IN URINE CULTURES

Intisar khlaif flaifel; Ahmed Abdulkareem  Ali; Ruqayah Qubtan  Taha; Mohammad  A. issa; Osama A.  mohsein; Hamza  A. Khalid

Intisar khlaif flaifel Al-Furat Al-Awsat University/ Babylon Technical Institutes/ Nursing Department
Ahmed Abdulkareem Ali University of Anbar, College of Education for Pure Sciences, Department of Biology
Ruqayah Qubtan Taha University of Anbar/ College of Medicine
Mohammad A. issa Head of the Microbiology Division, Yatta Health Directorate, Ministry of Health – Palestine
Osama A. mohsein Mazaya university collage, Department of Medical Laboratory techniques
Hamza A. Khalid Mazaya university collage, Department of Medical Laboratory techniques

Keywords: antibiotic, Sensitivity, bacilli

Abstract

Background; Urinary tract infections are common diseases, especially among women, and are caused by various types of bacteria, such as Proteus spp, Pseudomonas spp, and Staphylococcus spp. Study the extent of the spread of this bacterium in samples taken from patients in Thi-Qar Governorate plays an important role in knowing the causes of infection and methods of treatment for the purpose of reducing the incidence of infection and disease. There are various risk factors that can lead to the occurrence of urinary tract infections, such as the use of catheters, a lack of personal hygiene, or excessive use of antibiotics without consulting a doctor, which leads to antibiotic resistance problems in the future. Aims of the study: Providing healthcare and medical professionals with information about the spread of bacteria and the causes of therapeutic resistance in order to guide the most effective antibiotic treatment. Methodology: A retrospective study was conducted from April 10, 2023, to October 6, 2023, and a total of one thousand urine samples were collected. The samples were distributed equitably, with 500 urine samples collected for males and 500 urine samples collected for females. Age, nutritional status, and antibiotic usage were recorded without seeking medical consultation. The aforementioned samples underwent culture on blood agar and MacConkey agar. Various chemical tests were employed to identify and isolate bacteria, in addition to conducting a drug sensitivity test. utilizing an assortment of antibiotics under sterile conditions in the laboratory of Al-Habbobi Teaching Hospital. Results: The result show the Frequency and Percent of Pseudomonas Spp was Hight than Proteus spp and Staphylococcus spp. The frequency of AK was (173.0) was the higher Frequency among others antibiotics, And The frequency of AMS was (46.0), So the frequency of ATM was (44.0). And the result shows the frequency of AZM, CN, CRO, IMP, LEV and MEM was (41.0), CAZ, CTX, E, FBE (47.0), CIP, F (46.0), DO (62.0), FOX (87.0) and PRL (62.0). The result show the frequency of Pseudomonas Spp was higher in female than male (300.0 vs 200.0) respectively. While the frequency of Proteus spp and Staphylococcus spp was higher in male than female (150.0 vs 100.0). The frequency of AK was higher in male than female (89.0 vs 84.0). And the Frequency of AMS and F was 24.0 in female and 22.0 in male. And the frequency of ATM was equal in male and female (22.0). And the Frequency of AZM, CN, CRO, IMP, LEV and MEM was higher in male than female (21.0 vs 20.0). The result shows the frequency of CAZ, CTX, E and FEP was higher in female than male (24.0 vs 23.0), While CIP was similar in frequency in male and female (22.0). The frequency of DO and PRL was 32.0 in female and 30.0 in male. While the frequency of FOX was higher in male than female (44.0 vs 43.0). Conclusions: The results showed that the frequency of Pseudomonas ssp. was 500 (50%) and the frequency of Proteus spp. and Staphylococcus spp. was 250 (25.0%) for a total of 1000 urine samples. The results also showed that the frequency of amikacin (AK) was the highest among all antibiotics, followed by Fox at a percentage of 7.8%. The statistical results also showed that the frequency of Pseudomonas spp. was higher in women compared to men. On the contrary, the frequency of Proteus spp. and Staphylococcus spp. was higher in men than in women. When comparing drug sensitivity in men and women, the results showed that the frequency of amikacin (AK) and FOX in men is higher than in women.

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